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| | LinkBack | خيارات الموضوع | طريقة العرض |
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#1
2008-07-18, 04:15 PM
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| The Nucleic Acid Protocols Handbook بسم الله الرحمن الرحيم كتاب by Ralph Rapley الرابط او او Password is econiches باسورد لفك الملف المضغوط  |
| عدد 4 من الطلاب تقدموا بالشكر لكاتب الموضوع chemistry علي هذه المشاركة الطيبة : | ||
ibrahim matter (2008-07-20), saber_sciences (2008-10-08), Samo (2008-08-24), ابا عبدالله (2008-07-30) | ||
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#2
2008-07-20, 12:43 AM
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| رد : The Nucleic Acid Protocols Handbook جزاكم الله خيرا |
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#3
2008-07-30, 04:43 PM
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| رد : The Nucleic Acid Protocols Handbook الباسورد لا يعمل في فك الملف المضغوط |
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#4
2008-08-23, 11:13 AM
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| رد : The Nucleic Acid Protocols Handbook  By: Ralph Rapley(Editor) ISBN: 0896034593 ISBN-13: 9780896034594 Publisher: Humana Press - 2000-03-24 Hardcover | 1 Edition | 1072 Pages Table of Contents: Preface v Contributors xv Part I Nucleic Acid Extraction 1 Isolation of High-Molecular-Weight DNA from Animal Cells 3 2 Isolation of mRNA by Affinity Chromatography 9 3 Isolation and Purification of DNA from Plants 13 4 Purification of Uncontaminated, Intact Plant RNA 17 5 An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue 23 6 Isolating Chromosomal DNA from Bacteria 29 7 Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis 33 8 Isolation of Fungal Nucleic Acids 37 9 Total RNA Isolation from Bacteria 47 10 Simultaneous RNA and DNA Extraction from Biopsy Material, Culture Cells, Plants, and Bacteria 53 11 Spectrophotometric Analysis of Nucleic Acids 57 Part II Basic Separation and Analysis of DNA 12 Restriction Endonuclease Digestion of DNA 63 13 Agarose Gel Electrophoresis of Nucleic Acids 67 14 Preparation of RNA Dot Blots 71 15 Native Polyacrylamide Gel Electrophoresis 73 16 Southern Blotting of Agarose Gels by Capillary Transfer 77 17 Pulsed-Field Gel Electrophoresis 81 18 HPLC of DNA and PCR Products 105 Part III Probe Design, Synthesis, and Labeling 19 End-Labeling of DNA Fragments 117 20 Nick Translation and Random Hexamer Labeling of DNA 123 21 Generation of Labeled Probes by Polymerase Chain Reaction 127 22 Nonradioactive Oligonucleotide Probe Labeling 135 23 Preparation of Direct, Enzyme-Labeled DNA Probes 145 24 Random Prime Labeling of DNA Probes with Fluorescein-Tagged Nucleotides 149 25 Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Chemiluminescence 153 26 Hybridization of Enzyme-Labeled Probes and Detection by Chemiluminescence 157 27 Hybridization and Competition Hybridization of Southern Blots 163 28 Autoradiography and Fluorography 169 Part IV RNA Analysis Techniques 29 Formaldehyde Gel Electrophoresis of Total RNA 177 30 RNA Probes for the Analysis of Gene Expression 181 31 Primer Extension Analysis of mRNA 195 32 S1 Mapping Using Single-Stranded DNA Probes 201 33 Measurements of Rate of Transcription in Isolated Nuclei by Nuclear "Run-Off" Assay 207 34 One-Tube RT-PCR with Sequence-Specific RT Primers 213 35 Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA 219 36 Quantitative Analysis of RNA Species by Polymerase Chain Reaction and Solid-Phase Minisequencing 229 37 Nonradioactive Northern Blotting of RNA 239 38 Analysis of RNA by Northern Blotting Using Riboprobes 249 Part V Gene Library Construction and Screening 39 Production of Double-Stranded cDNA for Gene Library Synthesis 261 40 Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs 267 41 cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR 289 42 Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products 295 43 Subtraction Hybridization cDNA Libraries 305 44 Cloning Polymerase Chain Reaction Products Utilizing the T/A Overhang and a Kit 319 45 Extraction and Purification of Plasmid DNA 327 46 Biotinylated Probes in Colony Hybridization 333 47 Cloning Long Polymerase Chain Reaction Products 339 48 Cloning DNA Fragments in M13 Vectors 347 49 cDNA Library Construction for the Lambda ZAP-Based Vectors 355 50 Expression and Preparation of Fusion Proteins from Recombinant gt11 Phages 367 51 Antibody Screening of Bacteriophage gt11 DNA Expression Libraries 373 52 Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides 381 53 cDNA Library Screening with the Tetramethylammonium Chloride (TMAC) Technique Using Highly Degenerate Oligoonucleotide Probes 389 54 Screening Recombinant Libraries by Polymerase Chain Reaction 397 55 Construction and Screening of Cosmid Libraries 405 56 Generation of Large Insert YAC Libraries 415 57 YAC Library Storage and Transport 425 58 YAC Library Screening: Preparation of Hybridization Filters and Polymerase Chain Reaction Pools 431 59 YAC Library Screening: Hybridization and PCR-Based Screening Protocols 437 60 Phage-Display Libraries of Murine and Human Antibody Fab Fragments 449 Part VI DNA Sequencing 61 Preparation and Analysis of DNA Sequencing Gels 481 62 DNA Sequencing of Plasmids 489 63 Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors 493 64 Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction 499 65 Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products 505 66 Direct Polymerase Chain Reaction Sequencing with Denaturants 515 67 Direct DNA Sequencing of Polymerase Chain Reaction Products Using Magnetic Beads 523 68 Polymerase Chain Reaction Cycle Sequencing with Degenerate Primers 533 69 Direct Automated Cycle Sequencing of Polymerase Chain Reaction Products 541 70 Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products 547 71 DNA Sequencing by the Chemical Method 553 72 One-Step One-Lane Chemical Sequencing of DNA 557 Part VII Basic Polymerase Chain Reaction Methods 73 Polymerase Chain Reaction: Basic Principles and Routine Practice 569 74 Primer Selection and Design for Polymerase Chain Reaction 581 75 One-Step Optimization Using Touchdown and Stepdown Polymerase Chain Reaction 589 76 Cloning Gene Family Members Using Polymerase Chain Reaction with Degenerate Oligonucleotide Primers 595 77 Construction of Synthetic Genes by Polymerase Chain Reaction 609 78 Rapid Amplification of cDNA Ends 613 79 Multiplex Polymerase Chain Reaction 619 80 Inverse Polymerase Chain Reaction 625 81 Long Range Polymerase Chain Reaction 633 Part VIII Analyzing Genes, Mutations, and Protein Interactions 82 Nonradioactive Differential Display of Messenger RNA 645 83 Gene Isolation by Exon Trapping 653 84 DNA Rescue by the Vectorette Method 667 85 Random Amplified Polymorphic DNA (RAPDs) 675 86 Restriction Fragment Length Polymorphism 679 87 Detection of Mutations in DNA and RNA by Chemical Cleavage 685 88 Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols 695 89 Detecting Point Mutations by Denaturing-Gradient Gel Electrophoresis 705 90 Analysis of Nucleotide Sequence Variation by Solid-Phase Minisequencing 717 91 The Amplification Refractory Mutation System 723 92 DNase I Footprinting 729 93 Identification of Protein--DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference 737 94 The Gel Shift Assay for the Anmalysis of DNA--Protein Interactions 745 95 Yeast Two-Hybrid Library Screening 757 96 The Southwestern Assay 773 97 Nonradioactive Methods for the Detection of RNA-Protien Interaction 783 98 Tanscriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay 793 Part IX Mutagenesis, Transcription, and Translation In Vitro 99 Generating Nested Deletions with Exonuclease III 807 100 Primer-Directed Site-Specific Mutagenesis 815 101 Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template 827 102 Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates 835 103 Site-Directed Mutagenesis with LA-PCR Technology 845 104 Recombination and Mutagenesis by Overlap Extension PCR 857 105 Site-Directed Mutagenesis and Gene Fusion by Megaprimer PCR 865 106 Transcription In Vitro Using Bacteriophage RNA Polymerases 875 107 In Vitro Translation of mRNA in a Rabbit Reticuloctye Lysate Cell-Free System 885 108 In Vitro Translation of mRNA in a Wheat Germ Extract Cell-Free System 891 109 The Xenopus Egg Extract Translation System 895 110 Manipulation of Baculovirus Vectors 907 111 Procedures for the Analysis and Purification of His-Tagged Proteins 921 112 Detection and Immobilization of Proteins Containing the 6xHis Tag 935 113 Expression and Purification of Recombinant Proteins Using the pET System 947 Part X Gene Localization, Mapping In Situ, and Bioinformatics 114 Preparation of Tissue Sections and Slides for mRNA Hybridization 981 115 Use of Digoxigenin-Labeled Probes on Tissue Sections 985 116 Gene Mapping by Fish 991 117 Oligonucleotide Prins DNA Synthesis 1011 118 Chromosome-Specific Prins 1017 119 In Situ PCR Amplification of Intracellular mRNA 1023 120 An Introduction to Bioinformatics 1031 Index Link Pass: genem@5ter |
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#5
2008-08-24, 01:39 AM
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| رد : The Nucleic Acid Protocols Handbook شكرا اخى الكريم  |
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| مواقع النشر (المفضلة) |
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مواضيع مشابهة للموضوع: The Nucleic Acid Protocols Handbook | ||||
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| Sony ACID Pro | مكتبة البرامج | مكتبة البرامج | 0 | 2008-03-03 10:56 AM |
| كتاب: Handbook of Acid-Base Indicators | inmyheart | الكيمياء التحليلية | 5 | 2008-02-04 10:14 AM |
| كتاب Network Protocols Handbook | أحمد | الشبكات | 11 | 2007-06-03 01:52 PM |
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