Melanoma Techniques and Protocols: Molecular Diagnosis, Treatment, and Monitoring, a broadly diverse group of researchers and clinicians offer not only reviews of the most important recent advances in melanoma oncology, tumor immunology, and pathology, but also state-of-the- art molecular techniques for probing its basic biology. These readily reproducible methods can be used to develop new diagnostic approaches, therapeutics, and status monitoring of patients suffering both early- and late-stage melanoma. With emphasis on the experimental details critical to experimental success, each method is described in step-by-step fahion by hands-on masters, often those who have developed hem in their own laboratories. Comprehensive and highly instructive, Melanoma Techniques and Protocols: Molecular Diagnosis, Treatment, and Monitoring offers both clinicians and basic scientists concerned with malignant melanoma both new clinical perspectives and the wide ranging experimental protocols essential for achieving more rapid breakthroughs in this near-epidemic disease
1. The Many Molecular Mysteries of Melanoma
Brian Nickoloff
pp. 3-14
2. Isolation of Tumor Suppressor Genes in Melanoma by cDNA Microarray
Yan Su; Jeffrey Trent
pp. 15-29
3. Molecular Characterization of Melanoma-Derived Antigens
Marten Visser; Markwin Velders; Michael Rudolf; W. Kast
pp. 31-70
4. Experimental Induction of Human Atypical Melanocytic Lesions and Melanoma in Ultraviolet- Irradiated Human Skin Grafted to Immunodeficient Mice
Carola Berking; Meenhard Herlyn
pp. 71-84
5. Dissection of Immunosuppressive Effects of Ultraviolet Radiation
Stephen Ullrich; David Schmitt
pp. 85-97
6. Antisense Oligonucleotides as Research Tools
Jennifer Taylor; Scott Cooper; Nicholas Dean
pp. 99-106
7. Genetic Testing in Familial Melanoma: Epidemiologic/ Genetic Assessment of Risks and Role ofCDKN2A Analysis
David Hogg; Ling Liu; Norman Lassam
pp. 109-122
8. Role of Molecular Biology in Diagnostic Pathology of Melanoma
Andrzej Slominski; Andrew Carlson; Jacobo Wortsman; Martin Mihm
pp. 123-163
9. Identification of Altered Gene _Expression Associated with Pigmentary Lesions by Differential Display Analysis
I. Le Poole; Thomas Brown
pp. 165-179
10. Fluorescence In Situ Hybridization as a Tool in Molecular Diagnostics of Melanoma